PCNA positive cell in human hepatoma and liver cirrhosis.The comparison with BrdU - anti-BrdU method.
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چکیده
منابع مشابه
Cell proliferation in the gastrulating chick embryo: a study using BrdU incorporation and PCNA localization.
Cell proliferation in the gastrulating chick embryo was assessed using two independent techniques which mark cells in S phase of the mitotic cycle: nuclear incorporation of bromodeoxyuridine (BrdU) detected immunocytochemically and immunolocalization of proliferating cell nuclear antigen (PCNA). Computer-reconstructed maps were produced showing the distribution of labelled nuclei in the primiti...
متن کاملProliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique.
S-phase cells in intact animals of the coelenterate species Eirene viridula, Hydractinia echinata, Hydra attenuata, and Hydra magnipapillata incorporate the thymidine analogue bromodeoxyuridine (BrdU) into newly synthesized DNA. BrdU-labelled nuclei divide and cells appear to undergo normal differentiation. Whole-mount preparations and macerated tissues were screened for S-phase cells by means ...
متن کاملImmunohistochemistry and BrdU-LTR assay
The procedures for bone and bone marrow section preparation, immunostaining conditions and antibodies are described in Supplementary Methods. The procedure for BrdU pulse labeling, LTR and subsequent detection has been reported. The mice were fed BrdU (0.8 mg ml in water) for 10 days, during which time 40% of LT-HSCs would divide at least once. Seventy days after BrdU labelling, sections were s...
متن کاملApplication of Brdu in Studying Postnatal Neurogenesis
INTRODUCTION In the mammalian central nervous system (CNS), neurogenesis occurs mainly prenatally but continues in restricted regions during the postnatal life. It is intensive during the first two weeks after birth picking at P7-P9. Thereafter, neurogenesis is significantly diminished and remains longlife in the subventricular zone (SVZ) of the lateral ventricle and the subgranular zone (SGZ) ...
متن کاملPurification of nascent DNA chains by immunoprecipitation with anti-BrdU antibodies.
Commercially available monoclonal antibodies against 5bromodeoxyuridine (BrdU) have been widely applied recently for cytological detection of replicating DNA (Gratzner,H. (1982) Science 218, 474-475). Here we show that they can also be used for preparative purification of newly replicated DNA strands from the vastly larger fraction of genomic DNA. Anti-BrdU antibodies (Becton-Dickinson) specifi...
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ژورنال
عنوان ژورنال: Kanzo
سال: 1993
ISSN: 0451-4203,1881-3593
DOI: 10.2957/kanzo.34.771